LN-18人胶质母细胞瘤(STR鉴定正确)

价 格:¥2400

期 货:现货(冻存管发2支)

保藏中心:BTCC

资源编号:BTCC-1438

产地:中国

套 餐:

T25/复苏细胞 无血清冻存液 专用完全培养基500ml 南美特级胎牛血清500ml

详细资料说明书下载质检报告(COA)

Cell line nameLN-18
SynonymsLN 18; LN18; LN018
AccessionBTCC-1438
Resource Identification InitiativeTo cite this cell line use: LN-18 (RRID:BTCC-1438)
CommentsPart of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 72 hours (PubMed=7211194); 33 hours (PubMed=9842975).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Cell surface.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Brain, right temporal lobe; UBERON=UBERON_0002809.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (PubMed=9842975).
  • Mutation; HGNC; HGNC:8976; PIK3CB; Simple; p.Glu1051Lys (c.3151G>A); Zygosity=Homozygous (Cosmic-CLP=1240168; DepMap=ACH-000819).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys238Ser (c.713G>C); ClinVar=VCV000376575; Zygosity=Homozygous (PubMed=7732013; PubMed=10416987; PubMed=14614447; Cosmic-CLP=1240168; DepMap=ACH-000819).
HLA typingSource: PubMed=26589293
Class I
HLA-AA*02:01,24:02
HLA-BB*35:03,51:01
HLA-CC*04:01,14:02
Class II
HLA-DQDQA1*03:02,05:02

DQB1*04:02,04:02
HLA-DRDRB1*14:05,15:01
Genome ancestrySource: PubMed=30894373

Origin% genome


African0.83
Native American0.22
East Asian, North3.7
East Asian, South0
South Asian0.94
European, North53.19
European, South41.12
DiseaseGlioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of originHomo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cellMale
Age at sampling61Y
CategoryCancer cell line
STR profileSource(s): ATCC=CRL-2610; Cosmic-CLP=1240168; PubMed=22570425; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO12
D2S133817,20
D3S135815,16
D5S81811,13
D7S8208,10
D8S117912,14
D13S31712,13
D16S53911,13
D18S5117,19
D19S43312,15
D21S1128
FGA19,23
Penta D11
Penta E7,10
TH019
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Publications

PubMed=7211194; DOI=10.1007/BF00697180
Diserens A.-C., de Tribolet N., Martin-Achard A., Gaide A.-C., Schnegg J.-F., Carrel S.
Characterization of an established human malignant glioma cell line: LN-18.
Acta Neuropathol. 53:21-28(1981)

PubMed=7693337
Li H., Hamou M.-F., de Tribolet N., Jaufeerally R., Hofmann M., Diserens A.-C., Van Meir E.G.
Variant CD44 adhesion molecules are expressed in human brain metastases but not in glioblastomas.
Cancer Res. 53:5345-5349(1993)

PubMed=8509230; DOI=10.1002/ijc.2910540329
Rimoldi D., Romero P., Carrel S.
The human melanoma antigen-encoding gene, MAGE-1, is expressed by other tumour cells of neuroectodermal origin such as glioblastomas and neuroblastomas.
Int. J. Cancer 54:527-528(1993)

PubMed=7732013; DOI=10.1073/pnas.92.9.3963; PMCID=PMC42082
Flaman J.-M., Frebourg T., Moreau V., Charbonnier F., Martin C., Chappuis P.O., Sappino A.-P., Limacher J.-M., Bron L., Benhattar J., Tada M., Van Meir E.G., Estreicher A., Iggo R.D.
A simple p53 functional assay for screening cell lines, blood, and tumors.
Proc. Natl. Acad. Sci. U.S.A. 92:3963-3967(1995)

PubMed=9842975; DOI=10.1002/(SICI)1097-0215(19981218)79:6<640::AID-IJC15>3.0.CO;2-Z
Weller M., Rieger J., Grimmel C., Van Meir E.G., De Tribolet N., Krajewski S., Reed J.C., von Deimling A., Dichgans J.
Predicting chemoresistance in human malignant glioma cells: the role of molecular genetic analyses.
Int. J. Cancer 79:640-644(1998)

DOI=10.1007/0-306-46861-1_11
Ali-Osman F.
Brain tumors.
(In book chapter) Human cell culture. Vol. 2. Cancer cell lines part 2; Masters J.R.W., Palsson B.O. (eds.); pp.167-184; Kluwer Academic Publishers; New York; USA (1999)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x; PMCID=PMC8098486
Ishii N., Maier D., Merlo A., Tada M., Sawamura Y., Diserens A.-C., Van Meir E.G.
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=14614447; DOI=10.1038/sj.onc.1207198
Wischhusen J., Naumann U., Ohgaki H., Rastinejad F., Weller M.
CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death.
Oncogene 22:8233-8245(2003)

PubMed=14655754; DOI=10.1111/j.1750-3639.2003.tb00479.x; PMCID=PMC8095903
Bahr O., Rieger J., Duffner F., Meyermann R., Weller M., Wick W.
P-glycoprotein and multidrug resistance-associated protein mediate specific patterns of multidrug resistance in malignant glioma cell lines, but not in primary glioma cells.
Brain Pathol. 13:482-494(2003)

PubMed=17595512; DOI=10.1159/000104150
Rieger J., Frank B., Weller M., Wick W.
Mechanisms of resistance of human glioma cells to Apo2 ligand/TNF-related apoptosis-inducing ligand.
Cell. Physiol. Biochem. 20:23-34(2007)

PubMed=21406405; DOI=10.1158/0008-5472.CAN-10-3112
Grzmil M., Morin P. Jr., Lino M.M., Merlo A., Frank S., Wang Y.-H., Moncayo G., Hemmings B.A.
MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.
Cancer Res. 71:2392-2402(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314; PMCID=PMC4404347
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:e0121314.1-e0121314.22(2015)

PubMed=26496030; DOI=10.18632/oncotarget.6171; PMCID=PMC4791243
Patil V., Pal J., Somasundaram K.
Elucidating the cancer-specific genetic alteration spectrum of glioblastoma derived cell lines from whole exome and RNA sequencing.
Oncotarget 6:43452-43471(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers)ATCC; CRL-2610
BCRJ; 0420
Cell line databases/resourcesCLO; CLO_0007362
MCCL; MCC:0000287
cancercelllines; CVCL_0392
Cell_Model_Passport; SIDM00685
Cosmic-CLP; 1240168
DepMap; ACH-000819
LINCS_LDP; LCL-1353
Lonza; 1681
Anatomy/cell type resourcesBTO; BTO_0002005
Biological sample resourcesBioSample; SAMN03471122
BioSample; SAMN10988197
CRISP screens repositoriesBioGRID_ORCS_Cell_line; 410
Chemistry resourcesChEMBL-Cells; CHEMBL4295405
ChEMBL-Targets; CHEMBL4296458
GDSC; 1240168
PharmacoDB; LN18_839_2019
PubChem_Cell_line; CVCL_0392
Encyclopedic resourcesWikidata; Q54902768
Experimental variables resourcesEFO; EFO_0006636
Gene expression databasesArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM326196
GEO; GSM397661
GEO; GSM397662
GEO; GSM887269
GEO; GSM888344
GEO; GSM1374620
GEO; GSM1670048
Polymorphism and mutation databasesCosmic; 849858
Cosmic; 1746957
Cosmic; 1995484
Cosmic; 2302322
Cosmic; 2367489
Cosmic; 2491101
IARC_TP53; 2570
LiGeA; CCLE_635
Progenetix; CVCL_0392

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